GENERAL INFORMATION
Established:  1999
Ownership:  Private

Core business

Tools for research in molecular biology and medicine.

Employees:  9
Main Markets:  Bio medical & Agro Research companies.

Overview

GENE BIO-APPLICATION LTD., GeBA, develops novel tools for
research in molecular biology and medicine.

GeBA `s first product: GeBAflex-tube, Gel Extraction & Dialysis
kit, was completed in December 2001. GeBAflex-tube patent
protected unit is able to extract any biologic macromolecule from
any gel matrix and to dialyze small volume samples. This bio lab
consumer product is recognized as a serious challenger to
worldwide well-established competitors. GeBAflex-tube is produced
at the GeBA plant as a complete kit for macromolecule extraction
and dialysis process. The product has attracted over 20
distributors from New Zealand to the USA and 2 OEMs of worldwide
reputation.

GeBA's second project: Automated Plasmid Constructor (APC) for
high throughput cloning is destined to reduce cloning operations
from present typical duration of several months down to few
hours. The "Automated Plasmid Constructor" can effectively
combine several DNA fragments and ensure their correct
orientation. Engineering of vectors will be straightforward and
easy using Orientation Enrichment Reaction (OER), a breakthrough
automated technology in constructing plasmid for high throughput
cloning. OER is GeBA patent pending reaction. In addition, a
major benefit of this novel reaction is the ability to manipulate
DNA sequences, to construct any kind of mutation (point mutation,
deletions, insertions and others) as needed, in one single step
within a very short time. The whole process is completed by the
APC using the OER. The user provides only primers and the DNA
template. The first prototype is able to construct four different
genetic constructs in just five hours. The APC contains
disposable parts to eliminate any cross-contamination (DNA
templates, PCR products or PCR primers) during the process.
Moreover, the APC serves as a "mini-laboratory" performing
several independent processes, such as PCR, DNA fragment
extraction from gels, gel analysis of the size and quantity of
DNA or RNA fragments, and image processing. It is also possible
to carry out two or more of these processes jointly and
automatically. The APC harbors a partly disposable detection and
fragment extraction (DFE) unit (GeBA developed), which provides
the biologic processing capacity, OER, to be fully automated; the
aim of the unit is to detect and extract the precisely wanted DNA
fragment from the separating matrix. The DFE unit consists of
hardware and software that control the operating of the DFE unit;
analyzing the gel images, detecting and extracting the desirable
DNA fragment from the gel. The software (GeBA developed) runs
under Windows.
The need for such a robot is very important. In research, the
bottleneck of almost any molecular or cell biology research is
the construction of new vectors. In the future, gene therapy will
be a major tool used in the medical community. The biotechnology
industry (medicine and agriculture) will use more and more
genetically engineered organisms to produce new lines of
products.
The GeBA-APC is a fully functional operating system and provides
cloning services to GeBA customers in the world.

GeBA is now looking for ONE major strategic partner who will
invest capital for unit design and industrialization and
worldwide support of qualified engineers network.